phenotypic and genotypic characteristics of listeria monocytogenes isolated from dairy and meat products

Authors

abbas bahador department of microbiology, faculty of medicine, iran university of medical sciences, tehran, ir iran

behrooz sadeghi kalani department of microbiology, faculty of medicine, tehran university of medical sciences, tehran, ir iran

firuzeh valian department of microbiology, faculty of medicine, ilam university of medical sciences, ilam, ir iran

gholamreza irajian department of microbiology, faculty of medicine, tehran university of medical sciences, tehran, ir iran; department of microbiology, faculty of medicine, iran university of medical sciences, tehran, ir iran. tel: +98-2188058649, fax: +98-2188058649, e-mail:; lida lotfollahi, department of microbiology, faculty of medicine, urmia university of medical sciences, urmia, ir iran. tel: +98-9144470366, fax: +98-2188058649

abstract

conclusions mlva analysis showed that samples obtained from different sources could have similar genetic profile. as a result, administration of penicillin in patients with listeriosis (especially pregnant women) and antibiotic susceptibility test are recommended. the fast and accurate methods such as mlva for tracking of pollution sources of l. monocytogenes are recommended during outbreaks. results a total of 24 l. monocytogenes isolates were collected from the dairy and meat products. resistance of isolated l. monocytogenes strains to penicillin g were 54.54% (from dairy products) and 46.15% (from processed meat). genetic relatedness of isolates were assessed by mlva. out of 13 different types, type 2 with 6 strains and type 3 with 4 strains, were the most common types. materials and methods a total of 317 dairy products and meat-processed samples were collected. antibiotic susceptibility test was performed on each sample by the disk diffusion method (kirby bauer). five reference loci were used for typing of l. monocytogenes strains by mlva (multiple locus vntr analysis) technique. background listeria monocytogenes is a foodborne pathogen and a serious threat to the public health in the world. consumption of traditional foods such as dairy and meat products can be a major reason for relative abundance and isolation of these bacteria. objectives the purpose of this study was to determine the phenotypic and genotypic characteristics of l. monocytogenes strains isolated from dairy and meat products.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Isolation of Listeria monocytogenes from Meat and Dairy Products

Introduction: This study was intended to determine the presence and distribution of Listeria monocytogenes in various meat and dairy products from Qazvin Province by culture followed by biochemical and morphological assays. The identity of the isolates was further obtained by amplification of prfA gene in bacteria isolates. This gene is a transcriptional activator of virulence gene ex...

full text

Phenotypic and Genotypic Characteristics of Methicillin-Resistant Staphylococcus aureus Isolated from Dairy and Meat Products in Iran

Background: Methicillin-Resistant Staphylococcus aureus (MRSA) plays an important role in gastrointestinal diseases. The goal of this research was to determine phenotypic and genotypic characteristics of MRSA isolated from dairy and meat products in Iran. Methods: Ninety-three S. aureus isolates were prepared which had been obtained in our previous study. Antimicrobial susceptibility testing w...

full text

isolation of listeria monocytogenes from meat and dairy products

introduction: this study was intended to determine the presence and distribution of listeria monocytogenes in various meat and dairy products from qazvin province by culture followed by biochemical and morphological assays. the identity of the isolates was further obtained by amplification of prfa gene in bacteria isolates. this gene is a transcriptional activator of virulence gene expression w...

full text

Isolation of Listeria monocytogenes from Meat and Dairy Products

Listeria spp. are Gram-positive and facultative anaerobic bacteria, with a low G+C content in their genetic material. The genus comprises six species, namely Listeria monocytogenes, Listeria ivanovii, Listeria seeligeri, Listeria innocua, Listeria welshimeri, and Listeria grayi. The only pathogenic species of this genus is L. monocytogenes, although there have been few reports on the capability...

full text

Phenotypic and Genotypic Characterization of Atypical Listeria monocytogenes and Listeria innocua Isolated from Swine Slaughterhouses and Meat Markets

In the last decade, atypical Listeria monocytogenes and L. innocua strains have been detected in food and the environment. Because of mutations in the major virulence genes, these strains have different virulence intensities in eukaryotic cells. In this study, we performed phenotypic and genotypic characterization of atypical L. monocytogenes and L. innocua isolates obtained from swine slaughte...

full text

Prevalence and Antibiotic Susceptibility of Listeria Monocytogenes Isolated from Retail Ready-to-Eat Meat Products in Gorgan, Iran

Background and Objectives: Listeria monocytogenes are known as an emerged foodborne pathogen and considered as a severe health risk. In the present study, prevalence and antibiotic resistance of L. monocytogenes isolated from ready-to-eat meat products in Iran were assessed. Materials and Methods: A total of 200 ready-to-eat meat products, including chicken meat (wing, breast, and leg), lamb a...

full text

My Resources

Save resource for easier access later


Journal title:
avicenna journal of clinical microbiology and infection

جلد ۲، شماره ۳، صفحات ۰-۰

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023